Mueller Hinton Agar – 500 g – Oxoid
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1 Bottle × (1 × 500 g bottle of dehydrated powder)
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Mueller Hinton Agar – 500 g – Oxoid
Oxoid Mueller Hinton Agar (CM0337B) is a dehydrated culture medium that serves as the global standard for Antimicrobial Susceptibility Testing (AST), including the Kirby-Bauer disc diffusion method. It is also recommended for isolation of Neisseria and Moraxella spp. The standardised, low-inhibitor formulation delivers reproducible results compliant with both EUCAST and CLSI guidelines. Dissolve 38 g in 1 litre of distilled water and autoclave at 121 °C for 15 minutes; each 500 g bottle yields approximately 13 litres of prepared medium.
Specifications
- Size
- 500 g (dehydrated powder)
- Country of origin
- المملكة المتحدة (United Kingdom)
- Brand
- Oxoid (Thermo Scientific)
- Form
- Dehydrated / powdered culture medium
- Sterile
- No – requires preparation and autoclaving
- Storage
- Store powder at room temperature, away from light and moisture
- Application
- Antimicrobial Susceptibility Testing (AST); isolation of Neisseria spp. and Moraxella spp.
- Composition
- Beef extract, casein hydrolysate, starch, agar
- Preparation
- Dissolve 38 g in 1 L distilled water; autoclave 121 °C / 15 min
- Catalog code
- CM0337B
- Approximate yield
- ~13 litres per 500 g bottle
- Standards compliance
- EUCAST, CLSI (Kirby-Bauer disc diffusion)
Questions & answers
What is the primary use of Oxoid Mueller Hinton Agar 500 g?
Its primary use is Antimicrobial Susceptibility Testing (AST) by the internationally recognised Kirby-Bauer disc diffusion method. It is also used for isolating Neisseria and Moraxella species.
How is the medium prepared from the dehydrated powder?
Dissolve 38 g of powder in 1 litre of distilled water, then autoclave at 121 °C for 15 minutes. Each 500 g bottle yields approximately 13 litres of ready-to-use medium.
Is Oxoid Mueller Hinton Agar compliant with CLSI and EUCAST standards?
Yes. The product is specifically designed and manufactured to comply with both the American CLSI and European EUCAST standards for antimicrobial susceptibility testing.
What is the recommended storage temperature for this product?
The dehydrated powder should be stored at room temperature, away from light and moisture. Once prepared, the medium should be refrigerated until use.
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