Name: Mueller Hinton Agar – 500 g – Oxoid
Brand: Oxoid
SKU: mueller-hinton-agar-500-g-oxoid

Mueller Hinton Agar – 500 g – Oxoid

Oxoid™ Mueller Hinton Agar (Dehydrated) is the internationally recognised standard medium for Antimicrobial Susceptibility Testing (AST) using the Kirby-Bauer disc diffusion method. Manufactured to conform to both EUCAST and CLSI guidelines, it contains starch to absorb toxic by-products and support reliable bacterial growth. Each 500 g bottle of dehydrated powder prepares approximately 13 litres of ready-to-pour medium.

Specifications

Size: 500 g (dehydrated powder)
Country of origin: المملكة المتحدة (United Kingdom)
Brand: Oxoid (Thermo Scientific)
Form: Dehydrated / powdered culture medium
Sterile: No (requires preparation and autoclaving)
Storage: Store powder at room temperature, away from light and moisture
Application: Antimicrobial Susceptibility Testing (AST); isolation of Neisseria spp.
Composition: Beef extract, casein hydrolysate, starch, agar
Preparation: Dissolve 38 g in 1 litre of distilled water; autoclave at 121°C for 15 minutes
Catalog code: CM0337B
Approximate yield: ~13 litres per 500 g bottle
Standards compliance: EUCAST, CLSI (Kirby-Bauer disc diffusion)

Questions & answers

What is Mueller Hinton Agar (Oxoid) mainly used for?

It is primarily used for Antimicrobial Susceptibility Testing (AST) via the Kirby-Bauer disc diffusion method, conforming to EUCAST and CLSI standards. It is also used for the isolation of Neisseria species.

How much medium does the 500 g bottle prepare?

38 g is dissolved per litre of distilled water, meaning a 500 g bottle prepares approximately 13 litres of ready medium.

Is the medium supplied sterile?

No. It is supplied as a dehydrated (non-sterile) powder that must be dissolved in distilled water and sterilised by autoclaving at 121°C for 15 minutes.

Does this medium comply with international standards?

Yes. It is manufactured to comply with the European EUCAST standard and the American CLSI standard, making it the globally accepted reference medium for antibiotic susceptibility testing.